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. 2011 Sep;85(18):9346–9358. doi: 10.1128/JVI.00723-11

Fig. 6.

Fig. 6.

Resistant SW982 subclone SW-R can be made susceptible by blocking IFN signaling. (A) Susceptibility of SW-S, SW-R, and SW-R after treatment with IFN-abrogating agents. Representative micrographs 0 hpi and 24 hpi (VSV-rp30a, MOI 5) are shown. Single-cell subclone cell lines of SW982, SW-S, and SW-R are VSV sensitive and VSV resistant, respectively. SW-R cells were treated with valproate (VPA), vaccinia B18R, or JAK-inhibitor 1 (JAK-I) starting at the indicated time points relative to infection. (B) qRT-PCR was used to measure mRNA of the four indicated interferon-stimulated genes in SW-S, SW-R, SW-R pretreated for 14 h with VPA, and SW-R pretreated with vaccinia B18R for 4 days. Triplicate samples were analyzed. Error bars, SEM. (C) Triplicate human glial cultures were exposed to fresh medium, fresh medium with 200 U/ml of IFN, or conditioned medium. Medium was conditioned for 1, 2, or 3 days on SW-R. After 6 h of exposure, glial cell RNA was harvested and analyzed for MxA mRNA. Error bars, SEM.