Fig. 6.

Coimmunoprecipitation of UL16 and gE from HSV-infected cell lysates. Vero cells were mock infected or infected with HSV at an MOI of 10. At 20 h postinfection, the cells were harvested, washed with PBS, and lysed with NP-40 buffer. Equal amounts of infected-cell lysate (based on the expression levels of UL16, gE, and VP5; data not shown) were subjected to immunoprecipitation (IP) using rabbit polyclonal antibodies against UL16 or gE for the indicated times and at three different temperatures: 4°C (A), room temperature (RT) (B), and 37°C (C). The collected molecules were analyzed by SDS-PAGE and immunoblotting with antisera specific for UL16 or gE.