Fig. 9.

Flotation analysis of UL16 in the presence or absence of gE. (A) Vero cells expressing UL16-GFP or deletion mutant UL16(1-155)-GFP, either alone or together with gE, were osmotically disrupted, and the ability of the proteins to float to the upper fractions of sucrose step gradients during centrifugation was examined. Representative immunoblots are shown. The tops and bottoms of the gradients are indicated, along with the direction of flotation (arrows). (B) Densitometric analysis was used to quantitate the immunoblots, and the results are shown as the percentage of floating protein (top three fractions) relative to the total protein (all fractions). The averages of three experiments are shown, along with the standard deviations.