Fig. 7.

Identification of 68 cellular binding partners to PA-PB1 (A) and 34 to the 3P (B) complex. (A) A Venn diagram illustrates cellular proteins identified in affinity-purified complexes with PA (set 1 and set 2) or PA-PB1 (set 1). Most of the proteins that copurified with PA (Set 1 and set 2) also copurified with the PA-PB1 complex from the same virus. Numbers in the various boxes/sectors denote numbers of unique cellular proteins identified in each category. Highlighted in the lower panel of this figure are the 68 cellular proteins that interacted with the H5N1 PA-PB1 heterodimer. The boxes at the bottom of the figure denote proteins that were detected only in complexes not treated with nuclease (−N), only in complexes treated with nuclease (+N), or regardless of nuclease treatment (−/+N). (B) Venn diagram, representing cellular proteins identified in affinity-purified complexes with PA alone or PA-PB1 or the complete heterotrimeric polymerase complex (3P) from the A/Vietnam/1203/2004 (H5N1) virus. Most of the proteins identified in association with the 3P complex from A/Vietnam/1203/2004 were also detected in complex with PA plus PA-PB1. However, 34 proteins associated uniquely with the 3P complex. Numbers in the various boxes/sectors denote numbers of unique cellular proteins identified in each category. Highlighted in the lower panel of this figure are the 34 cellular proteins that interacted uniquely with the H5N1 3P. The boxes denote proteins that were detected only in complexes not treated with nuclease (−N), only in complexes treated with nuclease (+N), or regardless of nuclease treatment (−/+N). An asterisk next to the protein symbol depicts proteins previously reported to associate with the influenza virus polymerase. The proteins were grouped according to their functions. Proteins that are underlined were also detected in the corresponding PA-PB1 or 3P (H5N1) fraction from set 2.