Fig. 4.
Quantitation of infectious virus in secretions from prairie dogs infected with MPXV. Treatment with ST-246 reduces infectious virus in secretions. Prairie dogs were infected on day 0 with 3.8 × 105 PFU, and swab samples were collected every 2 days over a period of 30 days. Animals began receiving vehicle alone on day 3 (A, B, and C) or ST-246 at rash onset (D, E, and F), on day 0 (G, H, and I), or on day 3 (J, K, and L). Infectious virus was recovered from oral swabs (A, D, G, and J), ocular swabs (B, E, H, and K), and anal swabs (C, F, I, and L) by tissue culture. The number of PFU was quantitated by plaque counts, and average values of PFU per milliliter and standard deviations were plotted on a log scale as a function of day postinfection (A, B, C, G, H, I, J, K, and L) or day post-rash onset (D, E, and F).