Fig. 6.

Production of anti-OPXV antibodies in prairie dogs infected with MPXV. Initial detection and strength of antibody production in prairie dogs infected with MPXV produced different immune profiles dependent on ST-246 treatment regimen. Prairie dogs were infected with 3.8 × 10⁵ PFU, and serum was collected from blood drawn every 2 days over a period of 30 days. Animals began receiving vehicle alone on day 3 (A) or ST-246 at rash onset (B), on day 0 (C), or on day 3 (D). Serum was diluted 1:100 and tested against whole, inactivated vaccinia virus, and antibody production was measured by ELISA. Test serum was considered positive if its absorbance value was above the COV, which was determined by averaging OD measurements of BSC-40 cell lysates and adding 2 standard deviations.