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Evaluation of drug resistance in posttreatment virus isolates from prairie dogs by comet reduction. Approximately 50 PFU from swab isolates, VACV-IHDJ, and ST-246-resistant VACV-N267D was inoculated onto confluent monolayers of BSC-40 cells. Cultures were incubated with or without ST-246 for 2 (IHDJ and N267D) or 3 days (all PD isolates) and fixed with PBS containing formalin. Plaques and comets were visualized by IHC.
