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RFX is essential for CIITA recruitment and MHC-II expression. (A) Pull-down assays were performed with the wild-type promoter and extracts from SJO cells or SJO cells transfected with RFX5. A total of 2% of the input extract (−) and proteins purified by the pull-down assay (+) are shown. (B) MHC-II expression was analyzed by flow cytometry in SJO cells (open profile) and complemented SJO cells (gray profile).
