Fig. 2.
Effect of GAPDH mutants on TBSV repRNA replication based on CFE assay. (A) Schematic representation of GAPDH (Tdh2p) mutants expressed as GST fusion proteins in E. coli and affinity purified. Deleted regions are indicated by dotted lines. (B and C) Nondenaturing PAGE analysis of the 32P-labeled repRNA products obtained using GAPDH-depleted CFE (B) or CFE containing E. coli GAPDH (C) (all three yeast TDH genes are deleted, and E. coli GAPDH is expressed). Equal quantities of purified recombinant GAPDH mutants were added to each assay. Each experiment was repeated three times. See further details in Fig. 1A.