Table 1.

Relative plaque sizes of ¹³¹ACA¹³³ deletion-related mutants^a

Virus	Intended effect of mutation on SL4	Viable	Sequencing of 5′UTR	Plaque size ± SD (mm)	Relative plaque sizeb
SL4-E1-P1	Deletion of 131ACA133	Yes	Deletion of 131ACA133	1.55 ± 0.11	0.51*
SL4-E1-P5	Deletion of 131ACA133	Yes	Deletion of 131ACA133	2.67 ± 0.10	0.88
SL4-E2-P1	Deletion of 131ACA133	Yes	Deletion of 131ACA133	1.43 ± 0.07	0.47*
SL4-E6-P1	Deletion of 131ACA133	Yes	Deletion of 131ACA133 and insertion of AAAAA	2.50 ± 0.10	0.82
5Ainsert-SL4E	Deletion of 131ACA133 and insertion of AAAAA	Yes	Deletion of 131ACA133 and insertion of AAAAA	2.66 ± 0.09	0.88
SL4-E8-P1	Deletion of 131ACA133	Yes	Deletion of 131ACA133 and deletion of C80	2.83 ± 0.08	0.93
ΔC-SL4E	Deletion of 131ACA133 and deletion of C80	Yes	Deletion of 131ACA133 and deletion of C80	2.98 ± 0.08	0.98
SL4-E2-P3	Deletion of 131ACA133	Yes	Deletion of 131ACA133 and insertion of UCUAA	1.83 ± 0.07	0.60*
UCUAAinsert-SL4E	Deletion of 131ACA133 and insertion of UCUAA	Yes	Deletion of 131ACA133 and insertion of UCUAA	1.94 ± 0.10	0.64*
ACA-UGU	131ACA133 change to 131UGU133	Yes	131ACA133 change to 131UGU133	2.90 ± 0.06	0.96
WT		Yes		3.03 ± 0.07	1.00

^aThis table lists only prototypical plaque isolates for each genotype.

^bAsterisks represent statistical significance (P < 0.001).