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Spike histochemistry of neuraminidase-treated M41 S1 and B1648 S1 on chicken tissues. (A) S1 proteins of M41 and B1648 were pretreated with Vibrio cholerae neuraminidase before they were applied to chicken trachea, lung, intestine, and kidney slides. (B) Lectin histochemistry using biotinylated MAA lectins on kidney tissues. (C) Kidney tissue was pretreated with VCNA to remove α2,3-linked sia before VCNA-treated B1648 S1 was applied. Binding is indicated by arrowheads.
