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In vitro growth characterization of chimeric viruses in DF1 cells. The growth characteristics of parental and chimeric viruses were determined by multicycle growth curve in DF1 cells infected with an MOI of 0.01 PFU/cell. The viral titers were determined by limiting dilution on DF1 cells and immunostaining with a polyclonal antibody raised against the respective N protein. Each bar represents the mean and standard error of the mean of duplicate samples.
