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Growth kinetics of parental and chimeric viruses in the brains of 1-day-old chicks. Ten 1-day-old SPF chicks were inoculated with 256 HA units (approximately 1.28 × 106 PFU) of parental or chimeric virus via the intracerebral route. Two birds in each group were sacrificed daily until 5 dpi. The virus titers in the collected samples were determined by limiting dilution in DF1 cells and immunostaining with a polyclonal antibody raised against the respective N protein.
