Fig. 8.

Bioluminescence and statistical analysis of total fluxes in mice that received prophylactic treatment with HuMAb compositions and were challenged with WRvFire or with IHD-J-Luc vaccinia virus. (A to H) Bioluminescence of infected mice. BALB/c mice were inoculated i.p. with HuMAb compositions C (green triangles), C1 (blue inverted triangles), C2 (orange diamonds), and F (gray squares) at 30 μg/animal or with PBS (black circles) and were challenged with WRvFire (A-D) or with IHD-J-Luc (E-H) viruses 2 days later. Animals were subjected to whole-body imaging for 10 days to record bioluminescence and calculate mean total flux ± SD in the nasal cavity (A and E), lungs (B and F), liver (C and G), and spleen (D and H). (I to P) Statistical analysis. Mean total fluxes were used to calculate the t statistic between groups of mice treated with compositions C (green bars), C1 (blue bars), C2 (orange bars), and F (gray bars) and PBS-treated mice. The dashed horizontal lines depict critical t values of 2.09 in the WRvFire challenge (10 to 12 mice per group) (I to L) and 2.28 in the IHD-J-Luc challenge (6 mice per group) (M to P).