Table 1.
Mannose-rich glycosylation pattern and GRFT-mediated enhancement of HIV-1 binding to b12 and b6
| HIV-1 envelope pseudovirus (subtype)a | Predicted N-linked mannose-rich glycosylation siteb |
Fold increase with GRFT at 0.2 μg/ml |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 230 | 234 | 241 | 262 | 289 | 295 | 332 | 339 | 386 | 392 | 448 | b12-coated plate | b6-coated plate | |
| PVO.4 (B) | x | x | 8.2 ± 2.1 | 11.1 ± 0.9 | |||||||||
| QH0692.42 (B) | x | x | 4.1 ± 1.5 | 13.5 ± 1.7 | |||||||||
| COT6.15 (C) | x | x | 4.5 ± 1.9 | 4.5 ± 1.8 | |||||||||
| Du151.2 (C) | x | x | x | 2.3 ± 0.04 | 1.5 ± 0.2 | ||||||||
| CAP239.G3J (C) | x | x | 1.5 ± 0.4 | 6.2 ± 1.2 | |||||||||
| COT9.6 (C) | x | 1.5 ± 0.06 | 3.9 ± 2.1 | ||||||||||
a
Viruses are ranked by subtype and according to their enhancement by GRFT.
b
Mannose-rich glycosylations were identified from the amino acid sequence of each envelope clone (32). Missing sites are marked by x.