Fig. 1.

MRV infection induces eIF2α phosphorylation in a strain- and cell-type-specific manner. Cos-7 (A), HeLa (B), or L929 (C) cells were mock infected or infected with MRV T2J or T3D. At 0, 6, 12, 18, and 24 h p.i., cells were harvested and proteins were separated on SDS-PAGE and transferred to nitrocellulose. The membranes were blotted with rabbit α-μNS polyclonal antibody, rabbit α-tubulin polyclonal antibody, and rabbit α-phosphorylated (P)-eIF2α polyclonal antibody, followed by goat α-rabbit IgG conjugated with AP. Bound AP conjugates were detected by chemiluminescence staining and quantified with Quantity-One software. Quantified amounts of phosphorylated eIF2α were divided by quantified amounts of α-tubulin to adjust for differences in gel loading, and then increases in eIF2α phosphorylation relative to time zero were calculated and are shown on the right.