Fig. 2.

MRV-infected cells do not contain SGs at late times p.i. Cos-7 (A), HeLa (B), and L929 (C) cells were mock infected (top row) or infected with MRV T2J (middle row) or T3D (bottom row). At 24 h p.i., the cells were fixed and immunostained with rabbit α-μNS poly- clonal antiserum (left column) and goat α-TIAR polyclonal antibody (middle column), followed by Alexa 594-conjugated donkey α-rabbit IgG and Alexa 488-conjugated donkey α-goat IgG. Merged images containing DAPI-stained nuclei (blue) are shown (right columns). Bars = 10 μm.