Fig. 4.

NS5B but not NS5A increases Pin1 expression. (A) (Left) Huh7.5 cells were either mock infected or infected with HCV Jc1 for 4 h. Total-cell lysates harvested at 4 days postinfection were immunoblotted with the indicated antibodies. (Center) Blot images were scanned, and protein pixel intensity was measured using ImageJ software. (Right) Total RNAs were extracted, and qPCR was performed to quantify the Pin1 mRNA level. Data from two independent experiments were quantified. The asterisk indicates a significant difference (P < 0.05) between mock-infected and HCV Jc1-infected cells. (B) (Left) Equal amounts of cell lysates harvested from either IFN-cured or subgenomic replicon cells were immunoblotted with the indicated antibodies. (Center) Blot images were scanned, and the protein pixel intensity was measured using ImageJ software. (Right) Total RNAs were extracted, and qPCR was performed to quantify the Pin1 mRNA level. Data from two independent experiments were quantified. Asterisks indicate a significant difference (P < 0.05) between IFN-cured and replicon cells. (C) Huh7 cells were transfected with either a vector, Myc-tagged NS5A, or Myc-tagged NS5B. At 48 h after transfection, protein expression was determined by immunoblot analysis.