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Substitution mutants C166A and C254A produce B, but not A or C, capsids. Vero cells were infected with KOS (WT), hr74 (null), C166A, or C254A as described in Materials and Methods. Infected cell lysates were subjected to sucrose density gradient sedimentation, and fractions (1, top; 24, bottom) were subjected to SDS-PAGE and immunoblot analysis. Blots were divided into strips and probed separately with anti-UL6 and anti-VP5 antibodies. The positions of A, B, and C capsids are shown.
