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. 1998 Mar 1;12(5):667–678. doi: 10.1101/gad.12.5.667

Figure 2.

Figure 2

 Ephrin-B1/Fc multimers recruit LMW–PTP to EphB1 and EphB2 complexes. EphB1 immunoprecipitates from HRMEC (left) or P19 cells (center) or EphB2 immunoprecipitates (from HRMEC, right) were recovered from cells exposed to no addition (NA), ORF/Fc or ephrin-B1/Fc (500 ng/ml) as either dimers (1−anti-Fc) or as preclustered multimers (+anti-Fc). Receptor complex immunoblots were analyzed to show receptor recovery (anti-EphB1), left and center, or anti-EphB2, right, receptor activation (130 kD, anti-pTyr), an 18-kD tyrosine phosphoprotein (anti-pTyr) that is recognized by antibodies to LMW–PTP (anti-LMW–PTP). The 25-kD band in all samples is immunoglobulin light chain from the immunoprecipitation. LMW–PTP is recruited to both EphB1 and EphB2 receptor complexes only by ephrin-B1/Fc multimers (+anti-Fc).