Fig. 7.

STAg fails to protect NK cell-depleted mice and does not increase lung IFN-γ levels. (A) Prior to infection, C57BL/6 mice were treated with PBS (virus alone, n = 6 to 8 mice), isotype control IgG (n = 6 to 8), or anti-NK1.1 antibody to deplete NK cells (n = 6 to 8). Mice were then infected with 10³ TCID₅₀ (1 MLD₅₀) HK/483 influenza virus, treated with STAg, and monitored for morbidity for 10 dpi. (B and C) At 5 dpi, lungs were isolated from 3 or 4 mice per group. Viral titers were determined by TCID₅₀ analysis (B), and lung IFN-γ levels were determined by ELISA (C). Results from 3 independent experiments are shown. Error bars represent SEM. Each point in panels B and C represents an individual mouse.