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Binding affinity measurements with isothermal titration calorimetry. (Top) Calorimetric measurements at 22°C with 30 injections of 10 μl ORF158L into the cell containing histone H3. (Bottom) Energy (kcal/mol) released during each injection. (A) Wild-type ORF158L and H3. (B) Wild-type ORF158L and H3/H4.
