Fig. 1.
Poliovirus (PV) translation becomes resistant to sodium arsenite (Ars) stress. (A and C) HeLa cells infected with PV were stressed with 0.5 mM Ars (+) or not stressed with Ars (−) for 30 min prior to the time points indicated (hpi, hours postinfection). Cells were then pulse-labeled for 30 min with Tran35S-label before analysis by SDS-PAGE and autoradiography. The numbers below the gel in lanes 3 to 6 indicate the percentage of translation in Ars-stressed lanes compared to unstressed conditions as determined by densitometry as a function of time. The migration positions of molecular mass standards (in kilodaltons) are shown to the left of the gel, and the migration positions of PV proteins are shown to the right of the gel. (B) Western blot analysis of PV-infected cells to determine the cleavage of G3BP and phosphorylation of eIF2α using specific antibodies as indicated for each panel. Values indicate percentage of maximal eIF2α phosphorylation. G3BPcp, G3BP cleavage product.