Fig. 3.
Enterovirus translation is resistant to stressors. (A to C and E) SDS-PAGE/autoradiographs of cell proteins labeled with Tran35S-label. (A) PV-infected HeLa cells were infected with PV and stressed with heat shock 30 min prior to labeling at the indicated time points. (B) PV-infected 293T cells were treated with 20 μM Sal003 or dimethyl sulfoxide (DMSO) vehicle for 90 min prior to 30 min pulse-labeling. (C) HeLa cells infected with CVB3 were stressed as described in the legend to Fig. 1. (D) Immunoblots showing phospho-eIF2α in the panels. (E) PV-infected cells pretreated with alpha interferon (IFNα) (1,000 U) for 16 h. Control infection without IFN at 4 h is shown.