Fig. 5.
Expression of eIF5B modulates PV translation under stress. (A) (Top) Schematic depicting the 3Cpro cleavage site on eIF5B. (Bottom) Gel showing kinetics of cleavage of endogenous eIF5B during PV infection in the presence or absence of Ars. frag., fragment. (B) 293T cells expressing wild-type eIF5B or cleavage-resistant eIF5BQ478E were infected and stressed with 0.5 mM Ars before pulse-labeling translating proteins and analysis by SDS-PAGE and autoradiography. (C) Stimulation of PV translation by eIF5B expression was quantified by densitometry of autoradiographs. Results are an aggregate of three independent experiments, and error bars indicate standard errors. (D) Immunoblot analysis of eIF2α and phosphorylated eIF2α. (E) HA-specific immunoblot indicating expression of HA-eIF5B transgenes.