Fig. 6.
Expression of eIF5B479-1220 stimulates PV translation. (A) 293T cells expressing control GFP or eIF5B479-1220 were infected with PV and stressed with 0.5 mM Ars for 30 min, and then translating proteins were pulse-labeled and analyzed by SDS-PAGE/autoradiography. (B) Densitometric analysis of three independent experiments showing stimulation of translation of PV RNA in the presence of eIF5B479-1220 with and without Ars treatment relative to controls (Cont) expressing GFP. Error bars indicate the standard errors. (C) Immunoblot analysis of cell lysates for HA-tagged eIF5B479-1220 and phospho-eIF2α (p-eIF2α) as indicated.