Fig. 7.
Expression of eIF5B479-1220 rescues PV IRES translation activity. (A) 293T cells expressing GFP or eIF5B479-1220 were electroporated with FL reporter RNAs and treated with 50, 100, and 200 μM Ars for 90 min, and then lysates were analyzed for FL activity. Data are an aggregate of three experiments, and error bars represent the standard errors. (B) Data are expressed as fold stimulation from eIF5B expression versus GFP expression. (C) Immunoblot analysis of eIF5B479-1220 expression and phospho-eIF2α in 293T cells electroporated with FL reporters. Blots used eIF5B-specific antibody and phospho-eIF2α antibody as indicated.