FIGURE 1.

Schematic diagram showing the ROS excitation of the Acridan Lumigen PS-3 by ROS. Diagram showing the exchange of electrons between acridan Lumigen PS-3 and reactive species (ROS/RNS) in the presence of H₂O₂. The intermediate acridanyl radical spontaneously decarboxylates into CO₂ and excited N-methyl acridone, which emits light at 430 nm in the form of chemiluminescence. This diagram was modified from Wilson et al.²⁸ and the Amersham ECL Plus Western blotting detection kits (GE Healthcare), product booklet (codes: RPN2132 and RPN2133), http://gehealthcare.com/lifesciences.