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. 2011 Sep;22(3):95–107.

FIGURE 4.

FIGURE 4

Measurement of ROS using the Acridan Lumigen PS-3 and NBT assays in the xanthine-XO in vitro reaction. Diagram representing comparison of levels of ROS measured by NBT (line graph presenting absorbance values, primary y-axis) and Acridan Lumigen PS-3 (bar graph presenting photon counts, secondary y-axis). Both assays showed an increase in ROS levels with increasing concentrations of xanthine. The NBT assay showed a linear increase of 4.6-, 7.2-, and 11.1-fold in absorbance in 500, 700, and 1000 μM xanthine, respectively, compared with 0 μM, whereas the Acridan Lumigen PS-3 assay showed a dramatic 105- and 195-fold increase in photon counts in 700 and 1000 μM xanthine, respectively, compared with 0 μM. Results shown are representative of three independent experiments, and each column represents the mean and sd. *P: significance between 0 and 500 μM; **P: significance between 0 and 700 μM; ***P: significance between 0 and 1000 μM.