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. 2011 Jul 5;157(1):132–146. doi: 10.1104/pp.111.178681

Figure 4.

Figure 4.

AKR2A displays differential binding to members of CI cytosolic sHsps. Various sHsps, four CI cytosolic sHsps and one peroxisomal sHsp, were expressed in E. coli as GST fusion proteins. GST-tagged sHsps (3 μg) or GST alone (3 μg) immobilized onto glutathione agarose beads were incubated with His:AKR2A or His:GFP (1 μg), and proteins were precipitated with the beads. The precipitates were analyzed by western blotting using anti-His antibody. Subsequently, the membrane was stained with Coomassie blue.