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. 2011 Jul 14;157(1):147–159. doi: 10.1104/pp.111.181891

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Figure 3. — Chloroplast morphology in the atToc75-III↓ and AtOEP80↓ RNAi lines. A, Representative electron micrographs of wild-type, atToc75-III↓, and AtOEP80↓ chloroplasts. Cotyledons from 10-d-old plants of the indicated genotypes grown on MS-dexamethasone medium were analyzed. Approximately 10 whole-chloroplast micrographs from each of at least three independent plants per genotype were analyzed prior to selecting the images shown. Scale bar = 2.0 μm. B, Quantification of chloroplast electron micrographs. The length and width of each chloroplast in each of the micrographs described in the legend to A was measured. These values were used to estimate chloroplast cross-sectional area, shown in the left chart, and chloroplast shape (the length/width ratio), shown in the right chart. Values shown are means (±se) derived from measurements of 24 to 27 different chloroplasts per genotype.