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. 2011 Jul 13;157(1):160–174. doi: 10.1104/pp.111.180422

Figure 2.

Figure 2.

Characterization of the independent FOX lines expressing bZIP11 cDNA from pools 33, 93, and 70. A, Trehalose resistance is a dominant trait, as shown for lines 33-1 and 93-1. P, Seedlings from the parental lines: WT, the wild type; 33-1, FOX line 33-1; 93-1, FOX line 93-1. F1 represents the first generation from FOX line crosses with the wild type. B, PCR amplification of the FOX cDNA using DNA template from plants of lines 70, 128, 89, 93-1, and 33-1. MW, Molecular weight marker λ Pst1. C, Expression of bZIP11 in 14-d-old seedlings from FOX lines 70 and 93, the line expressing the E. coli trehalase treF (treF), and wild-type Col-0. D, Expression of the TRE1 trehalase in the genotypes from C. E, Seedlings with altered expression of bZIP1 do not grow on medium with 100 m trehalose (tre) compared with sorbitol (sorb). bZIP1oe and bZIP1as, bZIP1-overexpressing and antisense lines, as described by Kang et al. (2010). F, Expression of bZIP1 in seedlings of FOX lines 70 and 93, the line expressing treF, and the wild type grown on medium with sorbitol or trehalose. Expression was determined by Q-PCR and is given relative to PP2A (At1g13320). Error bars represent sd of three replicates.