Figure 3.

The roles of trehalase (TRE1) and of uORF2 in the FOX lines growing on trehalose. Error bars represent sd of three replicates. A, Expression of TRE1 in wild-type Col-0 (WT) and seedlings from two different plants of the tre1-1 line (Salk 147073c). B, Trehalase activity in flowers from the wild type and several plants from the tre1-1 line (P1–P4). C, tre1-1 and tre1-2 seedling growth compared with their respective wild types. Growth was on MS medium without (MS) or with 25 and 50 mm trehalose (25 mm T and 50 mm T). D, Genotype analysis of long root seedlings in the F2 generation of the cross 93-1 with the wild type. DNA from the wild type and 11 different seedlings (1–10) was used as the template. PCR was carried out to amplify the wild-type sequence of TRE1 (WT TRE1) or the T-DNA insertion at the TRE1 locus (KO TRE1) on the top agarose gel (tre1). PCR was also carried out to amplify the FOX cDNA on the bottom gel (Fox cDNA). E, Unlike on Suc, translational repression of bZIP11 does not occur on trehalose. Seedlings expressing the 5′ mRNA uORFs of the bZIP11 mRNA fused to the GUS gene were grown for 7 d on MS medium, transferred for 48 h to medium with Suc (SUC) or trehalose (TRE) at 0, 20, or 100 mm (0, 20, and 100), and then stained for GUS activity.