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. 2011 Jun 30;157(1):242–255. doi: 10.1104/pp.111.180893

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Figure 2. — E3 ubiquitin ligase activity assay of OsDIS1 and the RING finger mutant proteins. A, Scheme of OsDIS1 C3HC4 RING finger composition and the mutated amino acid in the RING finger. B, E3 ubiquitin ligase activity of OsDIS1. GST-OsDIS1 and its mutant form GST-OsDIS1(H71Y) fusion proteins were assayed for E3 activity in the presence of wheat E1 (GI: 136632), human E2 (UBCH5b), and 6xHis tag ubiquitin (Ub). The numbers at left denote the molecular masses of marker proteins in kilodaltons. RT represents the reaction time in the ubiquitination assay. GST was used as a negative control. Samples were resolved on a 10% SDS-PAGE gel. Nickel-horseradish peroxidase was used to detect His tag ubiquitin (top panel). The bottom panel indicates the presence of GST-OsDIS1/GST-OsDIS1(H71Y) proteins in the assay using anti-GST antibody. [See online article for color version of this figure.]