Skip to main content
. 2011 Jul 13;157(1):269–278. doi: 10.1104/pp.111.181669

Figure 1.

Figure 1.

Phenotypic characteristics and Pi concentration analysis of the Osphf1 mutants. A, Seedlings of the wild-type and three Osphf1 allelic mutants (phf1-1, phf1-2, and phf1-3) after growth in solution containing 30 μm As for 5 d. B, Growth performance of wild-type and Osphf1 allelic mutants grown in a solution culture with 200 μm Pi for 30 d. C and D, Cellular Pi concentration in the leaves (C) and roots (D) of wild-type and Osphf1 mutants. The data represents the mean of three replicates with sd indicated by error bars. E, RT-PCR analysis of the expression of Pi-responsive genes in wild-type and Osphf1-1 mutant roots. RNA was prepared from the roots of the 15-d-old seedlings grown in a solution culture with 200 μm Pi. The primers specific to the genes analyzed are listed in Supplemental Table S3. The transcript level of the Actin gene after 26 PCR cycles is used as a control.