Figure 2.

 Both p80 and p95 bind to telomerase RNA. A mobility shift assay was used to test the interaction of telomerase proteins with in vitro-transcribed T. thermophila telomerase RNA. (Lane 1) Radiolabeled RNA probe (10 nm) in the absence of protein. The p80 (lanes 2–5), p95 (lanes 6–9), or p80/p95 complex (lanes 10–13) was titrated by twofold increases from 40 to 320 nm with a constant amount of RNA (10 nm). This experiment included unlabeled nonspecific competitor 5S RNA at 2 μm. Some Tetrahymena telomerase RNA transcription reactions produced two mobility forms of telomerase RNA; results were the same with RNA transcription reactions yielding RNA of only one mobility.