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. 2011 Jul 5;157(1):317–327. doi: 10.1104/pp.111.180224

Figure 6.

Figure 6.

Transcriptional feedback regulation of the BX pathway by DIMBOA. Apoplastic infiltration with BX-inducing concentrations of chitosan (0.2%; A) and exposure to the volatile BX precursor indole (B) repressed Bx gene expression. BX-deficient bx igl double mutant lines displayed enhanced Bx gene transcription compared to BX-producing igl single mutant lines (C and D). Apoplastic leaf infiltration with DIMBOA (E and F) repressed Bx gene expression, whereas HDMBOA-glc had no effect (G), suggesting transcriptional feedback regulation by apoplastic DIMBOA. Shown are average fold-change values (±sem) of genes with a statistically significant level of induction (red) or repression (green) compared to mock treatments (A, B, E, and G) or BX-producing igl single mutant lines (C and D). Differences in expression between three biologically replicated samples from independent experiments were tested for statistical significance, using Student’s t tests or a nonparametric Wilcoxon-Mann-Whitney test when values did not follow normal distributions (α = 0.05). n.d., Not determined.