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. 2011 Jul 13;157(1):341–354. doi: 10.1104/pp.111.178582

Figure 4.

Figure 4.

Ectopic expression of AtJMT in 35S-jmt and 35S-jmt/ir-mje has profound, but differential, effects on spatiotemporal accumulation patterns of jasmonates in lamina, midribs, and petioles. Mean (±sd, n = 5) of jasmonate levels—OPDA, JA, MeJA, JA-Ile, and OH-JA (the sum of 12- and 11-OH-JA)—in wild-type, 35S-jmt-1, and 35S-jmt/ir-mje-1 leaves after OS elicitation (W + OS). Leaves were harvested 0 to 5 h after elicitation, dissected into leaf lamina, midvein, and petiole (see schematics above line graphs), and analyzed separately. Different jasmonates attained their maximum levels in different tissues postelicitation. MeJA hyperaccumulation occurred at the expense of JA-Ile and JA accumulation but only retarded the accumulation of OH-JA. AtJMT ectopic expression also had different consequences on the accumulation of JA-Ile and other JA-amino acid conjugates (Supplemental Fig. S4). Asterisks (35S-jmt-1) and plus signs (35S-jmt/ir-mje-1) represent significant differences between wild-type and transgenic lines (unpaired t test; */+ P < 0.05, **/++ < 0.001, ***/+++ < 0.0001).