Figure 6.

Alterations in jasmonate pools and relative composition are tissue dependent and most pronounced in petioles. A, Hierarchical clustering analysis performed, using the Pearson correlation as metric, on tissue-specific jasmonate signatures using vectors defined by the different levels reached at the different sampling times by a given jasmonate in a given tissue. Jasmonate profiles in systemic petioles relate the most to the signatures elicited after OS elicitation (W + OS) of treated leaves. The structure of locally elicited jasmonate signatures in wild type is not retained in 35S-jmt-1 and 35S-jmt/ir-mje-1 leaves as revealed by the clustering. B, Mean (±sd, n = 5) summed levels of the five most abundant jasmonates (OPDA, JA, MeJA, JA-Ile, and OH-JA) detected at 0 to 5 h after W + OS elicitation in local (top section) and systemic (bottom section) leaf tissues (lamina, midvein, and petiole) of wild-type, 35S-jmt-1, and 35S-jmt/ir-mje-1. Major quantitative changes in jasmonate pools were detected in midveins and petioles (Supplemental Fig. S6). Asterisks represent significant differences between tissues of wild-type and transgenic plants (unpaired t test; * P < 0.05, ** < 0.001, *** < 0.0001).