Figure 7.

SDS-PAGE and western blotting of 25-DAF cotton fiber protein with the SusC-specific antiserum. Crude extract was fractionated into soluble (sol), microsomal (micro), and plasma membrane (PM) fractions (see “Materials and Methods”), and the pellet (pell) remaining from the crude extract was washed extensively four more times in extraction buffer (w1, w2, w3, and w4; see “Materials and Methods”) in order to remove all residual loosely bound proteins. [See online article for color version of this figure.]