Table I. HPLC analysis of mucilage and cell wall monosaccharide composition from wild-type and mutant seeds.
Values shown are averages of three replicates ± se. n.d. Not determined.
| Sugar | Soluble Mucilage |
Whole Seed Enriched Cell Wall Fraction |
||||||
| Col-0 | cesa5-1 | cesa2-1cesa9-1 | cesa2-1cesa5-1cesa9-1 | Col-0 | cesa5-1 | cesa2-1cesa9-1 | cesa2-1cesa5-1cesa9-1 | |
| mg sugar g−1 seed | ||||||||
| Ara | 0.33 ± 0.096 | 0.50 ± 0.10 | 0.244 ± 0.012 | 0.26 ± 0.06 | 53.10 ± 1.73 | 62.87 ± 0.59 | 54.56 ± 1.96 | 62.94 ± 0.59 |
| Rha | 10.31 ± 0.41 | 12.48 ± 0.17a | 11.51 ± 1.52 | 12.56 ± 0.29a | 32.45 ± 2.17 | 22.20 ± 1.49a | 36.45 ± 1.99a | 22.41 ± 0.16 |
| Gal | 0.32 ± 0.11 | 0.29 ± 0.021 | 0.18 ± 0.023 | 0.14 ± 0.012 | 50.62 ± 0.80 | 53.82 ± 0.40 | 52.54 ± 1.18 | 50.04 ± 0.46 |
| Glu | 0.73 ± 0.18 | 0.57 ± 0.037 | 0.37 ± 0.069 | 0.516 ± 0.070 | 10.08 ± 0.30 | 7.65 ± 0.34a | 10.11 ± 0.12 | 7.06 ± 0.34 |
| Xyl | 1.05 ± 0.06 | 1.06 ± 0.040 | 1.06 ± 0.12 | 0.96 ± 0.062 | 27.14 ± 1.93 | 19.47 ± 2.65 | 26.31 ± 1.29 | 19.23 ± 0.92 |
| GalA | 9.21 ± 0.47 | 12.27 ± 2.14 | 9.50 ± 1.57 | 13.0 ± 2.04 | n.d. | n.d. | n.d. | n.d. |
Significantly different from Col-0 (P < 0.05).