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Functional characterization of oat putative lipoxygenases in E. coli. Hydroperoxy fatty acids were reduced to hydroxyl fatty acids by TnCl, followed by derivatization by trimethylsilyl as described in “Materials and Methods.” A, 18:2-9c,12c as substrate. B, 18:3-9c,12c,15c as substrate. The control is Rosetta2 cells transformed with the empty vector.
