Table I. The substrate specificity of AsPXG1 in P. pastoris.
The in vitro assays with different substrates were performed using cumene hydroperoxide as oxidant as described in “Materials and Methods.” The substrates for which no activity was detected are 18:0, 16:1-9t, 18:1-9t, and 12-OH-18:1-12c.
Substrate | Relative Activity |
% | |
14:1-9c | 76.3 |
15:1-10c | 98.8 |
16:1-9c | 88.9 |
17:1-10c | 94.1 |
18:1-6c | 87.3 |
18:1-9c | 100 |
18:1-11C | 71.9 |
19:1-10c | 77.0 |
20:1-11C | 74.8 |
22:1-13C | 54.8 |
24:1-15c | 52.6 |
18:2-9c,12c | 46.7 |
18:3-9c,12c,15c | 54.9 |