Table II. The kinetic parameters of oat AsPXG1 in P. pastoris on cooxidants.
The in vitro assays were performed using methyl oleate as substrate as described in “Materials and Methods.” Means with the same letters are not significantly different according to statistical analysis (Duncan test at P = 0.01, n = 3).
Substrate | Kmapp | Vmaxapp | Vmax/Kmapp |
μm | pkat·mg−1 | pkat·mg−1·μm | |
9-HPOD | 16.07 ± 0.78 a | 214.04 ± 11.84 e | 13.33 ± 0.92 g |
9-HPOT | 10.42 ± 0.42 b | 188.69 ± 22.88 e | 18.06 ± 1.46 h |
13-HPOD | 25.18 ± 1.80 c | 306.57 ± 22.02 e | 12.20 ± 0.92 g |
13-HPOT | 7.25 ± 1.52 b | 173.49 ± 23.51 e | 24.43 ± 4.54 i |
Cumene hydroperoxide | 215.76 ± 1.23 d | 883.34 ± 118.79 f | 4.10 ± 0.57 j |