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. 2011 Jul 22;157(1):454–463. doi: 10.1104/pp.111.178822

Table II. The kinetic parameters of oat AsPXG1 in P. pastoris on cooxidants.

The in vitro assays were performed using methyl oleate as substrate as described in “Materials and Methods.” Means with the same letters are not significantly different according to statistical analysis (Duncan test at P = 0.01, n = 3).

Substrate Kmapp Vmaxapp Vmax/Kmapp
μm pkat·mg−1 pkat·mg−1·μm
9-HPOD 16.07 ± 0.78 a 214.04 ± 11.84 e 13.33 ± 0.92 g
9-HPOT 10.42 ± 0.42 b 188.69 ± 22.88 e 18.06 ± 1.46 h
13-HPOD 25.18 ± 1.80 c 306.57 ± 22.02 e 12.20 ± 0.92 g
13-HPOT 7.25 ± 1.52 b 173.49 ± 23.51 e 24.43 ± 4.54 i
Cumene hydroperoxide 215.76 ± 1.23 d 883.34 ± 118.79 f 4.10 ± 0.57 j