Figure 6.

The effect of prolonged cold exposure on PmDAM expression in October. One-year-old branches were cut from cv Nanko and cv Ellching trees in October, artificially defoliated, and placed in a growth chamber for cold(+) treatment (7°C ± 2°C) or cold(−) treatment (25°C ± 3°C) under dark conditions. Vegetative buds were collected from the middle portions of branches at 0, 16, 32, or 64 d of cold(+) or cold(−) treatment. Gene expression was measured by real-time PCR as described in Figure 3. Transcript levels of each gene were normalized by PmUBQ. The means of three technical replicates are shown, with error bars representing sd. The means of another biological replicate are shown in Supplemental Figure S3. Changes in expression are shown as logarithmic graphs.