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Northern-blot analysis of plastid gene expression during amyloplast differentiation. Total RNA was extracted from BY-2 cells in D or B medium sampled every 12 h. Five micrograms of total RNA was loaded in each lane. Lane 1, Before inoculation (0 h); lanes 2 to 5, after inoculation in D medium (12, 24, 36, and 48 h after inoculation, from the left); lanes 6 to 9, after inoculation in B medium (12, 24, 36, and 48 h after inoculation, from the left). nt, Nucleotides.
