Figure 2.

Effects of inhibitors of plastid gene expression on starch accumulation within the plastids. A to C, Staining of starch granules in plastids. BY-2 cells were cultured for 48 h in B medium either in the absence (A) or presence of 100 mg L⁻¹ rifampicin (B) or 100 mg L⁻¹ spectinomycin (C) and stained with iodine solution. Bars = 20 μm. D and E, Accumulation of starch in BY-2 cells. Aliquots of cultured cells were sampled every 24 h and subjected to quantitative analysis. D, BY-2 cells were cultured in B medium in the absence (crosses) or presence of 50 mg L⁻¹ (triangles), 100 mg L⁻¹ (circles), or 150 mg L⁻¹ (squares) rifampicin. E, BY-2 cells were cultured in B medium in the absence (crosses) or presence of 100 mg L⁻¹ (triangles), 200 mg L⁻¹ (circles), or 300 mg L⁻¹ (squares) spectinomycin. Values shown are means ± sd (n = 3).