Table I. Expression levels of plastid genes in BY-2 cells cultured in B and D media.
Signal ratios (Cy5 to Cy3) of six independent spots for appropriate genes on tobacco plastid microarrays from two biological replicates were averaged, and all reliable data sets (coefficient of variation = sd/mean < 0.3) are listed. Spot identification (ID) and corresponding gene names follow Nakamura et al. (2003).
| Spot ID | Gene | Ratio of B to D (Mean ± sd) | Spot ID | Gene | Ratio of B to D (Mean ± sd) | Spot ID | Gene | Ratio of B to D (Mean ± sd) |
| 307 | ycf3(c)exon2 | 1.65 ± 0.28 | 303 | rps16(c)5′exon | 0.99 ± 0.07 | 320 | 3′rps12(c)intron | 0.85 ± 0.12 |
| 314 | clpP(c)exon2 | 1.47 ± 0.14 | 18 | trnG | 0.99 ± 0.17 | 142 | ycf2 | 0.83 ± 0.08 |
| 86 | atpB(c),atpE(c) | 1.37 ± 0.18 | 161 | rrn23 | 0.98 ± 0.19 | 194 | ycf1(c) | 0.81 ± 0.17 |
| 70 | rps4(c) | 1.31 ± 0.24 | 91 | psal | 0.98 ± 0.10 | 129 | rpl16 (c)3′exon | 0.81 ± 0.09 |
| 312 | clpP(c)3′exon | 1.31 ± 0.15 | 72 | trnT(UGU) (c) | 0.98 ± 0.10 | 155 | trnV(GAC) | 0.79 ± 0.20 |
| 164 | rrn5 | 1.26 ± 0.10 | 153 | 3′rps12(c),orf131 | 0.97 ± 0.07 | 323 | trnA intron | 0.78 ± 0.05 |
| 305 | ycf3(c)3′exon | 1.25 ± 0.06 | 89 | accD | 0.96 ± 0.07 | 145 | trnL(CAA) (c) | 0.78 ± 0.13 |
| 139 | ycf2 | 1.24 ± 0.12 | 35 | rpoC1 (c)5′exon | 0.96 ± 0.04 | 173 | rpl32 | 0.78 ± 0.03 |
| 162 | rrn4.5 | 1.24 ± 0.08 | 85 | atpE(c),atpB(c) | 0.95 ± 0.20 | 33 | rpoC1 (c)3′exon | 0.78 ± 0.21 |
| 25 | atpH(c) | 1.22 ± 0.14 | 113 | clpP(c) | 0.95 ± 0.14 | 83 | trnV(UAC) (c) | 0.77 ± 0.15 |
| 75 | trnL(UAA) | 1.20 ± 0.13 | 128 | rpl14(c) | 0.95 ± 0.17 | 135 | rpl2 (c)intron | 0.76 ± 0.07 |
| 62 | psaA (c) | 1.19 ± 0.35 | 15 | psbl | 0.94 ± 0.08 | 313 | clpP(c)intron2 | 0.76 ± 0.06 |
| 319 | rpl16(c)intron | 1.19 ± 0.16 | 61 | psaB(c) | 0.94 ± 0.25 | 140 | ycf2 | 0.73 ± 0.07 |
| 311 | trnV (c)intron | 1.15 ± 0.16 | 34 | rpoC1 (c)intron | 0.94 ± 0.13 | 31 | rpoC2(c) | 0.73 ± 0.11 |
| 110 | rps18 | 1.13 ± 0.05 | 54 | trnS(c) | 0.93 ± 0.06 | 193 | ycf1(c) | 0.71 ± 0.12 |
| 148 | ndhB(c)3′exon | 1.12 ± 0.13 | 97 | psbJ (c),orf99 | 0.93 ± 0.15 | 22 | atpF (c)intron | 0.70 ± 0.13 |
| 158 | trnl(GAU) | 1.12 ± 0.08 | 21 | atpF (c)3′exon | 0.92 ± 0.11 | 4 | trnK(c)3′exon | 0.70 ± 0.12 |
| 124 | rps11(c) | 1.10 ± 0.24 | 125 | rpl36(c) | 0.91 ± 0.13 | 131 | rps3(c),rpl22 | 0.69 ± 0.10 |
| 136 | rpl2(c)5′exon | 1.10 ± 0.13 | 93 | ycf4 | 0.91 ± 0.12 | 68 | trnS(GGA) | 0.68 ± 0.15 |
| 26 | (atpH(c)promoter) | 1.09 ± 0.16 | 308 | ycf3(c) intron 1 | 0.91 ± 0.07 | 130 | rpl16(c)intron,5′exon | 0.67 ± 0.10 |
| 52 | psbD, psbC | 1.07 ± 0.09 | 127 | rps8(c) | 0.91 ± 0.10 | 315 | clpP(c)intron1 | 0.67 ± 0.09 |
| 171 | ndhF,orf350 | 1.06 ± 0.14 | 134 | rpl2 (c)3′exon | 0.90 ± 0.16 | 166 | trnR | 0.67 ± 0.14 |
| 23 | atpF(c)5′exon | 1.04 ± 0.04 | 47 | trnE(c) | 0.90 ± 0.26 | 36 | rpoB(c) | 0.66 ± 0.06 |
| 28 | atpl(c) | 1.04 ± 0.10 | 30 | rpoC2(c) | 0.89 ± 0.10 | 96 | orf99 | 0.66 ± 0.08 |
| 32 | rpoC2(c) | 1.03 ± 0.26 | 112 | 5′rps12(c) | 0.88 ± 0.08 | 121 | petD 5′exon,intron | 0.65 ± 0.17 |
| 317 | petB intron | 1.02 ± 0.15 | 20 | atpA(c) | 0.88 ± 0.23 | 46 | trnY(c) | 0.61 ± 0.14 |
| 102 | petL | 1.01 ± 0.15 | 66 | orf74(c) | 0.88 ± 0.13 | 87 | rbcL | 0.60 ± 0.18 |
| 132 | rpl22,rps3(c) | 1.01 ± 0.21 | 100 | orf103(c) | 0.88 ± 0.17 | 3 | psbA(c) | 0.59 ± 0.09 |
| 65 | ycf3(c),orf74(c) | 1.01 ± 0.11 | 160 | rrn23 | 0.88 ± 0.10 | 322 | trnl intron | 0.58 ± 0.04 |
| 115 | psbB | 1.00 ± 0.17 | 126 | ψ infA(c) | 0.87 ± 0.19 | 103 | petG | 0.53 ± 0.07 |
| 13 | psbK | 1.00 ± 0.24 | 321 | 3′rps12 (c)5′exon | 0.87 ± 0.11 | 45 | trnD(c) | 0.52 ± 0.07 |
| 182 | ndhE(c) | 1.00 ± 0.11 | 191 | rps15(c) | 0.86 ± 0.13 | 56 | ycf9 | 0.51 ± 0.15 |
| 10 | rps16(c) | 1.00 ± 0.08 | 306 | ycf3(c)intron2 | 0.85 ± 0.04 | 156 | rrn16 | 0.51 ± 0.13 |
| 149 | ndhB(c)intron | 1.00 ± 0.20 | 63 | psaA(c) | 0.85 ± 0.02 | 141 | ycf2 | 0.44 ± 0.07 |
| 152 | rps7(c) | 0.99 ± 0.07 |