Fig. 6.

Leucine deprivation-induced fat loss is blocked in β-less (KO) mice. Wild-type and β-less mice were fed a control (cont) or (−) leu diet for 7 d. The energy expenditure was measured by indirect calorimetry in these mice over 24–48 h after 6 h of acclimation to the metabolic chamber. Data are mean ± sem for at least two independent experiments with mice maintained on each diet for each experiment (n = 6–10 for each group). Statistical significance was determined by ANOVA followed by the SNK test for the effects of either group vs. control diet-fed wild-type (WT) mice (*, P < 0.05), vs. (−) leu diet-fed wild-type mice (#, P < 0.05), or vs. control diet-fed β-less mice (&, P < 0.05). A, Adipose tissue mass in proportion to body weight. B, Body weight change. C, Daily food intake. D, Twenty-four-hour oxygen consumption. E, RER. F, Rectal temperature. G, Physical activity.