Figure 7.

Sqd protein associates with the translational repressor protein Bruno. (A) ³⁵S-labeled Bruno protein (lane 1) was incubated with a series of GST fusion proteins, and after incubation with glutathione-coated–agarose beads, Bruno’s association was assessed by SDS-PAGE and autoradiography. The following GST fusions were tested for their ability to bind Bruno: (lane 2) GST only; (lane 3) GST–SqdA; (lane 4) GST–SqdS; (lane 5) GST–SqdB; and (lane 6) GST–Encore. (B) To further investigate the interaction between Sqd protein and Bruno protein, coimmunoprecipitation experiments were done. Whole cell lysates from wild-type ovaries were subjected to immunoprecipitation with a mouse anti-Sqd monoclonal or with a mouse anti-Wind antisera. The blots were probed with a rabbit anti-Bruno antisera: (lane 1) whole cell ovarian extract; (lane 2) anti-Wind immunoprecipitate; (lane 3) anti-Sqd immunoprecipitate.